Along this route we can observe beautiful riparian ecosystems composed of arboreal species such as ash, alder, black poplar, willow, water-blood and elder, among others. Up the slopes, besides cork oaks and holm-oaks, we can also find trees such as the mastic tree, the cornflower, the aderno-bastardo and the tamagueira. The European orchids and the yellow lily of the mountains, more precisely the bulbous plant iris xiphium - Lusitanian subspecies, both rare in this region, should also be highlighted. The millenary olive trees will not go unnoticed when walking through these fields. The fauna here is very rich; on land, from otters to gineta, and in the air, from the osprey to the bee-eater, from the kingfisher to the kingfisher, the magpie, the porpoise or the rock blowtorch, among other birds, there are many species to discover, just a little attention. In this region you can find five different species of swallow: the tern, the tern, the tern, the rock-rumped swallow and the tern. But there is more: it is not at all impossible to be surprised by the griffon or even by the black stork, by day or, if you walk at night, by the imposing eagle owl, the discreet wood owl, the Galician owl or the grey and red-necked Nightjar.
Fish like the barbel, the boga, the fataça, are already swimming in the water, which are less easy to spot, unlike the beautiful "aquatic forests" composed of aquatic plants of the genus potamogeton among others, which can be seen at various points.
As far as geodiversity is concerned, several old folds and terraces from the origin of the river Tagus formation can be seen on this route. Of great relevance is the outcrop of rocks that in some points of this route can be around 650 million years old, being probably the oldest of the whole Mação County, integrated in the Ossa Morena Zone. Don't miss also the beautiful meanders in the Ribeira de Boas Eiras near Poço do Pombal.